Research Assistant III/IV - Developmental Biology - Mikedis Lab
Cincinnati Children's Hospital Medical Center
Location: Cincinnati, Ohio
Academic / Research
Internal Number: 140346
Expected Starting Salary Range: 22.79 - 27.77 SUBFUNCTION DEFINITION:Dr. Maria Mikedis' laboratory is seeking a postdoctoral Research Assistant who will investigate how spermatogonial stem cells/progenitors differentiate to form sperm.
Specific topics include how translational control regulates spermatogonial stem cells' differentiation into mitotically dividing progenitors (Mikedis, et al.,2020 eLife), and how post-transcriptional regulation of mRNA induces progenitors to transition from mitosis to meiosis (Soh, Mikedis, et al., 2017 PLoS Genetics). The Mikedis laboratory uses a multidisciplinary approach, including cutting edge tools in scRNA-seq, molecular biology, functional genomics, biochemistry, and mouse genetics.
Perform experiments and record data
Follow established research protocols. Understand the scientific principles involved in the research. Apply those principles to troubleshoot and modify procedures and techniques when appropriate. Maintain accurate and detailed records of all laboratory work and experiments. Analyze data and perform statistical and graphical analysis of data. Design and execute daily laboratory experiments in coordination with senior laboratory personnel. Apply standard protocols to specific experiments. Assists in training post doctoral fellows, graduate students, and junior laboratory personnel in laboratory techniques and equipment. Capable of directing an undergraduate trainee in simple, well-established procedures. Maintains an active role in laboratory's meetings, presentations, and publications. Contributes material for reports, publications, journal articles, and abstracts. Able to orally present data in group presentations.
Troubleshoot and correct erroneous results or problems with laboratory equipment. Troubleshoot and/or make modifications as necessary to established procedures. Maintain inventory of laboratory supplies and equipment. Maintain cell cultures. Operate standard laboratory equipment. Willing and able to assist in daily operations of the laboratory including answering phone, maintaining lab supplies, organization, and cleanliness. Maintains optimal protocol and assay accuracy by conducting regular quality checks. Provides appropriate safety monitoring checks as assigned (radiation safety, biosafety, etc.)
Research and improve current methods
Utilize internal and external resources to reproduce, evaluate, research, and improve techniques, bring in new techniques or modify existing techniques. Evaluate innovative techniques
Serve as a Resource
Demonstrate competence in specific knowledge, skills and behaviors. Effectively communicate with team members and contribute to their growth by sharing knowledge, experience, and skills. Stay abreast of changes in key systems. Serve as a resource to the department in specific area(s).
Maintain/manage strong working relationships with clients, colleagues, and other key stakeholders. Respond to requests quickly and effectively. Identify and understand customer needs and expectations and meets them. Communicate any relevant information to clients, colleagues, and other key stakeholders.
Required for RAIII:
Bachelor's degree in a related field
1 year of work experience in a related job discipline OR Master's degree in a related field
Required for RAIV:
Bachelor's degree in a related field
3 years of experience in related job discipline OR Master's degree and 2 years experience
Preferred: Candidates with expertise in some of the following areas are preferred: reproductive biology, biochemistry, functional genomics, bioinformatics, mouse models, and cell culture techniques.
Cincinnati Children's is proud to be an Equal Opportunity Employer that values and treasures Diversity, Equity, and Inclusion. We are committed to creating an environment of dignity and respect for all our employees, patients, and families (EEO/AA).
About Cincinnati Children's Hospital Medical Center
At Cincinnati Children’s, we come to work with one goal: to make children’s health better. We believe in a team approach, both in caring for patients and their families, and in advancing science and discovery. We strive to do better, and find energy and inspiration in our shared purpose. If you want to be the best you can be, you can do it at Cincinnati Children’s.