Staff position needed in laboratory whose primary focus is studying host-microbiota interactions during intestinal inflammation.
This position requires a highly motivated individual with a track record of success.
Basic requirements are listed below:
excellent oral and written communication skills
high quality record-keeping including ongoing maintenance of a well-structured laboratory notebook and laboratory inventory
excellent organization skills
drive, focus and ability to set an efficient and productive agenda after guidance
at least 2 years experience working in a microbiology laboratory
previous experience in handling mice and in mouse colony maintenance
Under moderate supervision, set up, conduct experiments, and continue research projects following protocols including the appropriate recording of data and reporting of standard or variant results. Assist in other laboratory and research functions.
Welcome to the Department of Pathology, Microbiology & Immunology at Vanderbilt University Medical Center. This website will give you a glance at our complex, multi-faceted department. We are proud of the cutting-edge research carried out by our faculty and trainees. The work ranges from basic science investigations in microbial pathogenesis, immunobiology and cancer biology to clinical and translational research defining the current and future practice of pathology. Our highly regarded educational programs include a highly sought after residency program in anatomic and/or clinical pathology, PhD granting graduate programs in microbe-host interactions and molecular pathology and immunology, and abundant opportunities for clinical or postdoctoral research training. We also now offer a highly innovative course for undergraduate students at Vanderbilt. Finally, we take great pride in providing state-of-the-art pathology services to the patients of Vanderbilt University Medical Center and beyond.
Day Shift/ Non-Exempt Position
POSITION: Full-Time, Non-Exempt
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This position is 50% mouse colony management, 20% laboratory maintenance and 30% laboratory research. General responsibilities include handling mice in both regular and gnotobiotic housing, maintaining gnotobiotic mouse colony and assisting with gnotobiotic mouse experiments. Laboratory techniques that will be performed are: dissection of the spleen, liver and intestines from mice post mortem, prepare RNA and DNA from tissues, perform genotyping PCR analysis from DNA isolated from rodent tissues and feces, quantitate and perform quality control of RNA and DNA isolated from rodent tissue, subclone DNA fragments into vectors, transform bacteria, plate bacteria for quantitation and quality control of gnotobiotic mouse colony, and work with bacterial samples in an anaerobic chamber. Laboratory maintenance duties include: organization and maintenance of the laboratory's breeding colony of genetically manipulated and gnotobiotic mice, assist in gnotobiotic mouse experiments, maintain records of daily activities and experimental results, prepare common-use chemical solutions for experiments, change gas cylinders for anaerobe chamber and assist with general lab cleaning duties including autoclaving, filling water containers and cleaning dishes.
Under moderate supervision, conduct and evaluate experiments and assays
Understand and follow experimental protocols (may include animal handling, tissue culture, etc.)
Record, compile, interpret, and communicate data/results
Follow University safety protocols (may include use of combustibles, radioactivity, toxic material, and/or carcinogens)
Perform calculations involving molarity, % solutions, and serial dilutions
Prepare, operate and troubleshoot equipment in experimental procedures
Read and follow all equipment manuals
Maintain and repair equipment according to manufacturer and University guidelines
Ensure Laboratory is prepared for experiments
Maintain sterile conditions as required
Demonstrate an understanding of acceptable procedures and techniques
Recognize problems and suggest solutions
Assist in modifications as directed
Provide appropriate written documentation of experiments
Prepare preliminary research progress reports
Summarize test results appropriately
Preferred Education, Skills, and Experiences:
Bachelor degree in Biological Sciences, Microbiology, Molecular Biology, Immunology or a related field.
Minimal of 2 years experience working in a microbiology laboratory setting.
Prior experience in mouse handling and husbandry (at least 2 years).
Prior experience in basic microbiology techniques such as plating bacteria.
Prior experience with PCR (real-time and conventional) and other basic molecular biology technology and sterile cell culture is highly desired.
Bachelor's Degree (or equivalent experience) and 2 years experience
Occasional: Standing: Remaining on one's feet without moving.
Occasional: Walking: Moving about on foot.
Occasional: Lifting under 35 lbs: Raising and lowering objects under 35 lbs from one level to another
Occasional: Push/Pull: Exerting force to move objects away from or toward.
Occasional: Bending/Stooping: Trunk bending downward and forward by bending spine at waist requiring full use of lower extremities and back muscles
Occasional: Reaching below shoulders: Extending arms in any direction below shoulders.
Occasional: Handling: Seizing, holding, grasping, turning or otherwise working with hand or hands.
Occasional: Vision: Clarity of near vision at 20 inches or less and far vision at 20 feet or more with depth perception, peripheral vision, color vision.
Occasional: Noise: May include exposure to occupational noise levels which equal or exceed an 8-hr time-weighted average of 85 decibels, requiring enrollment in VUMC's Hearing Conservation Program which includes training, use of hearing protection, and periodic audiometry.
Continuous: Communication: Expressing or exchanging written/verbal/electronic information.
Occasional: Chemicals and Gasses: Medications, cleaning chemicals, oxygen, other medical gases used in work area.
Occasional: Pathogens: Risk of exposure to bloodborne pathogens and other contagious illnesses.
Vanderbilt Health recognizes that diversity is essential for excellence and innovation. We are committed to an inclusive environment where everyone has the chance to thrive and to the principles of equal opportunity and affirmative action. EOE/AA/Women/Minority/Vets/Disabled
Vanderbilt University Medical Center is home to Vanderbilt University Hospital, The Monroe Carell Jr. Children’s Hospital at Vanderbilt, the Vanderbilt Psychiatric Hospital and the Vanderbilt Stallworth Rehabilitation Hospital. These hospitals experienced more than 61,000 inpatient admissions during fiscal year 2015. Vanderbilt’s adult and pediatric clinics treated nearly 2 million patients during this same period. Vanderbilt University Hospital and the Monroe Carell Jr. Children’s Hospital at Vanderbilt are recognized again this year by U.S. News & World Report’s Best Hospitals as among the nation’s best with 18 nationally ranked specialties. Vanderbilt University Medical Center is world renowned because of the innovation, work ethic and collegiality of its employees. From our health care advances to our compassionate care, Vanderbilt owes its accomplishments and reputation to staff and faculty who bring skill and drive and innovation to the medical center day after day. World-leading academic departments and comprehensive centers of excellence pursue scientific discoveries and transformational educational and clinical advances across the entire spectrum of health and disease.As t...he largest employer in middle Tennessee, we welcome those who are interested in ongoing development in a caring, culturally sensitive and professional atmosphere. Most of us spend so much of our lives at work, we want to be part of maintaining a workplace in which people support one another and encourage reaching for excellence. Many high-achieving employees stay at Vanderbilt because of the professional growth they experience and because of their appreciation of Vanderbilt’s benefits, public events and discussions, athletic opportunities, beautiful setting and, above all, sense of community and purpose.Vanderbilt and its employees share a set of mutual expectations that have been created with productivity, legality, fairness and safety always in mind. We believe that our investment in training and compensating employees multiplies in value when we enable individuals to deliver their best performance for the benefit of us all.